Isolation and Identification of a Red Yeast Strain from the Intestine of Zebrafish

Objective:The yeast was isolated from the wild zebrafish(Danio rerio) intestine.Method:The red yeast strain ZF-2 isolated from the intestine of zebrafish using potato dextrose agar(PDA) plates,then was identified by morphological and physiological characteristics.The 26S rDNA D1/D2 domian of the strain were amplified by PCR method,and then contrust a phylogenetic tree.Result:The strain ZF-2 had pink elliptical shape of yeast cells,and a polar budding pattern,which can assimilate glucose,galactose and sucrose,etc.The PCR product is 642bp,and the analysis of 26S rDNA sequence indicated that the strain ZF-2 was the closest to Rhodotorula slooffiae with 99%-100% sequence similarity.Construction of phylogenetic trees showed that the strains ZF-2 and Rhodotorula slooffiae(EU583485) belonged to one subcluster,thus the strain ZF-2 was identificated as Rhodotorula slooffiae.Conclusion:This is the first report on the Rhodotorula slooffiae from the gut of zebrafish.