Study on New Process of Enzymatic Synthesis for Structured Lipids

A research was done about lipase-catalyzed ester exchange reaction of soybeen oil with methyl caprylate under different conditions.The reaction constitutions were analyzed after the ester exchange reaction.The results showed that the best efficency of ester exchange reaction catalyze by Lipozyme RM IM were on the condition of enzyme dose 16%,reaction temperature 42 ℃,substate ratio 8∶1,reaction time 16 h,tert-butyl alcohol as solvent.Under the conditions,the incorporation of caprylic acid was up to 59.45 mol%.In addition,several long chain aliphatic acid methyl esters were detected in reaction products,such as methyl palmitate(14.58 moL%),methyl oleate(21.36 moL%),methyl linoleate(45.44 moL%),a spot of methyl stearate(5.28 moL%)and methyl linolenate(2.56 moL%),which were all the main compositions of biodiesel.High value-added biodiesel can effectively cut cost of lipase-catalyzed synthetical process of structured lipids and make it more feasible for commercial process.