Epigallocatechin-3-gallate( EGCG)reduces the LPS-induced injury of newborn rat primary cultured glial cells

Objective To determine the effects of EGCG on lipopolysaccharide( LPS)-induced neuroinflammation and investigate the role of neuroprotection mediated by EGCG. Methods Primary cultures of rat gliacyte were used as an in vitro model to examine the effects of EGCG on LPS-induced neuronal damage. The intracellular Glu and γ-GABA were quantified via HPLC. Then the protein level of TNF-α,IL-1 β and IL-8 was determined by ELISA and Western blot assay. Results Compared with the control group,LPS apparently induced the production of intracellular ROS( P 0. 05) and released the TNF-α,IL-1 β and IL-8 in the primary cultures supernatant( P 0. 05). Compared with the LPS group,EGCG significantly attenuated the release of TNF-α,IL-1 βand IL-8( P 0. 05) and the level of i NOS protein( P 0. 05). LPS apparently induced the production of intracellular ROS( P 0. 05) and released the TNF-α,IL-1 β and IL-8 in the primary cultures supernatant( P 0. 05). EGCG significantly attenuated the release of TNF-α,IL-1β and IL-8( P 0. 05) and the level of i NOS protein( P 0. 05),and rugulated the concentration of Glu/γ-GABA( P 0. 05). Conclusions EGCG is effectivein protecting hosts against LPS-induced neuroinflammation through anti-inflammatory effects and regulating extracellular Amino acid levels.