Effects of Fluoxetine and Tianeptine on the Expression of PKC in Hippocampus of Chronic Stress Depression Rats

Objective To investigate the effects of fluoxetine and tianeptine on the expression of PKC in hippocampus of chronic stress depression rats. Methods 60 male Wistar rats were randomly divided into depression group,fluoxetine group,tianeptine group and control group with each group 15 rats.The rats in depression group,fluoxetine group and tianeptine group were given stress stimulation for 21 days,and meanwhile rats in control group were kept normally.The rats in fluoxetine group were given fluoxetine by intragastric administration(10 mg/kg),rats in tianeptine group were given tianeptine by intragastric administration(50 mg/kg).While the depression group and control group were given same volume of 0.9% sodium chloride injection.The ethology examination was performed by using method of open-field and experiment of fluid consumption.The expression of PKC was detected by Western-blotting method. Results After the stress stimulation,horizontal crossing numbers,erection times,modification times and percentage of sacchar-consumption of the rats in depression group were significantly less than those of the control group(P0.01).The horizontal crossing numbers,the erection times,the modification times and the percentage of sacchar-consumption of the rats in fluoxetine group were significantly less than those of the depression group(P0.05).The percentage of sacchar-consumption of the rats in tianeptine group was significantly more than that of the depression group(P0.05).In the test with Western-blotting method,the expression level of PKC in the hippocampus in depression group was significantly lower than that of the control group(P0.01).The expression level of PKC in the hippocampus in fluoxetine group was significantly higher than that of the depression group(P0.01),but no statistically significant difference showed between fluoxetine group and control group.The expression level of PKC in the hippocampus in tianeptine group was significantly higher than that of the depression group(P0.01),but significantly lower than that of the control group(P0.01). Conclusion Fluoxetine can reverse the decrease of the expression of PKC in hippocampus of chronic stress depression rats while tianeptine can also partly have the same function.