The Impact of Local Injection of Tanshinone II-A on Osteoclast of Orthodontic Tooth of Rabbit

Objective: To observe the effect of local inject tanshinone Ⅱ-A on the osteoclast in the pressure side of periodontal membrane during orthodontic tooth movement. Methods: Twenty healthy and male rabbits were randomly divided into 5 groups( 4 rabbits each group) to copy the bilateral mandibular first molar movement model. 0. 36 mg / d tanshinone II A sulfonate was injected into the gingival mucosa of mesial surface of the left first molar once a day as experimental group while the same amount of normal saline was injected into the opposite molar of the same name as control group. The rabbits were killed at 1th,3th,7th,14 th,21thday after injection. The distance of bilateral mandibular first molar mesial movement was measured by plaster cast. The periodontal slice of the mandibular first molar was taken and conducted by HE staining and tartrate resistant acid phosphatase staining. The osteoclasts in the pressure side of periodontal membrane were counted under the light microscope. Results: Except for the frist day,the tooth movement distance of experimental group were significantly higher than that of control group( P 0. 05). Compared with control group,the number of osteoclasts significantly increased in experimental group( P 0. 05). Conclusion: In the process of orthodontic tooth movement,tanshinone Ⅱ-A can increase the number of osteoclasts,enhance cell activity and bone resorption intensity and improve the tooth movement.