贵州白香猪γ干扰素基因的克隆与序列分析
Guizhou Agricultural Sciences(2012)
Abstract
为获得贵州白香猪γ干扰素基因,以提取的经刀豆蛋白A诱导培养的贵州白香猪外周血淋巴细胞总RNA为模板,扩增全长猪γ干扰素基因,并克隆至pMD18-T Simple载体后测序分析。结果表明,pIFN-γ基因全长501bp,可编码166个氨基酸,其中前23位氨基酸为信号肽序列;pIFN-γ含有2个潜在N-糖基化位点;贵州白香猪与国内地方猪种之间pIFN-γ基因差异不大,具有很近的亲缘关系,其中与藏猪、成华猪、枫径猪、梅山猪的同源性达100%,而与内江猪的亲缘关系相对较远,与荣昌猪(DQ902588)亲缘最远。
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Key words
interferon gamma-γ,sequence analysis,gene clone,Guizhou Baixiang Pig
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