川牛膝多糖的分离、纯化及单糖组成

Chinese Journal of Appplied Environmental Biology(2003)

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Abstract
采用水提醇沉法提取川牛膝多糖粗品CPC,经685弱碱阴离子交换柱层析和Bio-Gel P2凝胶柱层析进一步纯化,得到川牛膝多糖RCP(refined Cyathula officinalis Kuan polysaccharide).紫外扫描、高效液相色谱、聚丙烯酰胺盘状凝胶电泳证明RCP为均一组分.质谱图显示RCP的分子量(Mr)主要分布在1 000~2 200.薄层正交试验确定了RCP完全水解的最优条件.薄层层析及高效液相色谱-蒸发光散射检测(HPLC-ELSD)技术揭示,RCP单糖组成为D-果糖和D-葡萄糖. 图11 表2 参18
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Key words
polysaccharide,monosaccharide composition,purification
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