液固两相法培养金龟子绿僵菌的研究

Journal of Fujian College of Forestry(2012)

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Abstract
通过对绿僵菌液固两相发酵技术的研究,优化控制发酵过程,筛选出玉米粉(2份)+麦麸(2份)+谷壳(2份)为固体发酵培养基的优化配方,液体种子发酵周期72 h,初始接种量控制在25%为宜。保持25-28℃的培养温度和83%-94%的相对环境湿度,同时选择合适的覆盖物(可降低杂菌的污染)。固态发酵周期11 d,菌粉干燥温度控制在35℃,时间24 h,可缩短生产周期。
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Key words
solid-submerge fermentation,conidia,Metarhizium anisopliae
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