Chondro-inductive injectable hydrogels for stem cell-based intervertebral disc tissue engineering

Event Abstract Back to Event Chondro-inductive injectable hydrogels for stem cell-based intervertebral disc tissue engineering Taryn Naidoo1, Jessica Frith1, Donna J. Menzies1, Clementine Pradal1, Andrew Cameron1, Ernst Wolvetang1, Lisbeth Grondahl2, Darryl Whitehead3, Stan Gronthos4, Andrew Zannettino4, Peter Ghosh5 and Justin Cooper-White1, 6, 7 1 University of Queensland, Australian Institute for Bioengineering and Nanotechnology, Australia 2 University of Queensland, School of Chemistry and Molecular Biosciences, Australia 3 University of Queensland, School of Biomedical Sciences, Australia 4 University of Adelaide, Centre for Stem Cell Research, Robinson Institute, Australia 5 Monash University, Monash Institute of Medical Research, Australia 6 CSIRO, Biomedical Manufacturing, Australia 7 University of Queensland, School of Chemical Engineering, Australia Stem cell-based therapies require easily manufactured, injectable biomaterials tailored for delivery to different tissue sites and at the same time, having the ability to impart directive cues to such cells. We report here on a bio-synthetic multicomponent hydrogel system that provides significant flexibility in terms of the available mechanical property slate, biofunctionality and utility as injectable materials. This new gel platform is made from a hydroxyphenol (HP) functionalised, highly branched poly(ethylene glycol) (PEG), with or without HP functionalized hyaluronic acid (HA), via an enzyme mediated, oxidative crosslinking method. The versatility of this hydrogel system was exemplified through variations in the degree of HP substitution, polymer concentration, type and concentration of crosslinking reagents, which resulted in a range of mechanical properties (0.1 – 25 kPa) and gelation kinetics (5 seconds – 10 minutes) for these gels. Co-crosslinking of the PEG-HP/HA-HP conjugates with full length Fibronectin (without needing to a priori modify them) and/or a sulfated polysaccharide (SP) encouraged attachment and spreading of human mesenchymal stem cells (hMSCs). Under in vitro long term culture conditions, the presence of the HA and SP was shown to induce chondrogenic gene upregulation and protein and GAG matrix secretion, in the absence of inductive media, but to inhibit osteogenesis (even in the presence of osteogenic media), in human MSCs (Figure 1). Under small animal model in vivo assessment, in-situ gelation and tissue integration (with and without hMSCs) was confirmed (Figure 2), along with evidence of ectopic chondrogenesis after 21 days. Successful outcomes from both in vitro and small animal model in vivo assessments confirm that this novel hydrogel system shows significant promise for cartilage tissue engineering applications. It is currently under long term assessment in a large animal (ovine) model of IVD disease, results of which will be presented. Keywords: Cell Differentiation, Hydrogel, Tissue Engineering, stem cell Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: New Frontier Oral Topic: Regenerative medicine: biomaterials for control of tissue induction Citation: Naidoo T, Frith J, Menzies DJ, Pradal C, Cameron A, Wolvetang E, Grondahl L, Whitehead D, Gronthos S, Zannettino A, Ghosh P and Cooper-White J (2016). Chondro-inductive injectable hydrogels for stem cell-based intervertebral disc tissue engineering. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02659 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Taryn Naidoo Jessica Frith Donna J Menzies Clementine Pradal Andrew Cameron Ernst Wolvetang Lisbeth Grondahl Darryl Whitehead Stan Gronthos Andrew Zannettino Peter Ghosh Justin Cooper-White Google Taryn Naidoo Jessica Frith Donna J Menzies Clementine Pradal Andrew Cameron Ernst Wolvetang Lisbeth Grondahl Darryl Whitehead Stan Gronthos Andrew Zannettino Peter Ghosh Justin Cooper-White Google Scholar Taryn Naidoo Jessica Frith Donna J Menzies Clementine Pradal Andrew Cameron Ernst Wolvetang Lisbeth Grondahl Darryl Whitehead Stan Gronthos Andrew Zannettino Peter Ghosh Justin Cooper-White PubMed Taryn Naidoo Jessica Frith Donna J Menzies Clementine Pradal Andrew Cameron Ernst Wolvetang Lisbeth Grondahl Darryl Whitehead Stan Gronthos Andrew Zannettino Peter Ghosh Justin Cooper-White Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.