Characterization Of Extracellular Amylase Produced By Haloalkalophilic Strain Kocuria Sp Hj014

The haloalkaliphilic bacterium Kocuria sp. (HJ014) has the ability to produce extracellular amylase. The aim of this study was to purify and characterize this protein. The amylase enzyme with a specific activity of 753,502 U/mg was purified 5.7- fold using Sepharose 4B and Sephacryl S-300 gel filtration columns. The molecular weight of the enzyme was 45,000Da as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The amylase showed maximum activity at pH 9 and 50 degrees C in the presence of 3.5M NaCl. The K-m was 3.0mg/ml and V-max 90.09 U/ml. It was found that extracellular amylase from Kocuria sp. has a high industrial potential.