Immobilization of flavan-3-ols onto sensor chips to study their interactions with proteins and pectins by SPR

Aude A. Watrelot,Dong Tien Tran,Thierry Buffeteau,Denis Deffieux,Carine Le Bourvellec,Stéphane Quideau,Catherine M.G.C. Renard

Applied Surface Science（2016）

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摘要

•Flavanol-macromolecule interactions were determined using SPR.•Flavanols were chemically modified with a linker bearing a thiol group.•Flavanols were immobilized onto a carboxymethyl dextran surface.•Citrus pectin interacted more with flavanols than apple pectin.•Epicatechin interacted more with BSA than flavanol oligomer.

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关键词

SPR,CM5,BSA,PLP,DP,ITC,EPI,CAT,DP2,DP8,STrt,SH,ATR,RU

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