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Extraction and purification of IgG by hydrophilic organic solvent salting-out extraction

Yuesheng Dong, Bofeng Pang,Fang Yu, LingLing Li,Wen-Cai Liu,Zhilong Xiu

Journal of Chromatography B（2016）

Cited 16|Views1
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Abstract
•The distributions of IgG in different salting-out extraction systems were investigated.•The optimized SOES in extraction of IgG is ethanol/K2HPO4 system.•The ethanol/K2HPO4 SOES do not cause any obvious change in the structure of IgG.•We provide a new alternative for the separation and purification of immunoglobulin.
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Key words
Active retention,Immune globulin,Purification,Recovery,Salting-out extraction systems,Structural constant
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