Rapid Extraction Of Dna Suitable For Ngs Workflows From Bacterial Cultures Using The Pdqex

Background: PDQeX is a novel, single-step DNA extraction method that purifies nucleic acid from sample in under 30 min.Materials & Methods: Six bacterial suspensions from species with different cell morphologies and growth optima were made. DNA from half the suspension was purified using PDQeX and the other half using a conventional column purification method. Sequencing and analyses using Ion PGM were performed, blinded to extraction method and species.Results: Genomes extracted with either method sequenced successfully. No significant sequence distribution biases were evident between PDQeX and column purification. Surveyed community preference suggested comparable performance between the two extraction methods.Conclusion: DNA prepared using the PDQeX performs as well for whole-genome sequencing as DNA purified using a conventional method, albeit much more rapidly.METHOD SUMMARYWe introduce the PDQeX: a new, rapid, single-step method for DNA extraction that is compatible with high-throughput sequencing. Sample is added to the thermoresponsive PDQeX extractor tube with a cocktail of thermostable enzymes. Upon incubation and heating the extracted DNA is expelled into a collection tube ready for use in downstream applications in under 30 min.