Effect on the expression of VEGF and HIF-1 alpha in atherosclerosis rat myocardial with oral HSP60 (618.8)

Objective to study the effect on the expression of vascular endothelial growth factor (VEGF) and hypoxia inducing factor ‐ 1 alpha (HIF ‐ 1 alpha) in atherosclerosis(AS) rat myocardial with oral heat shock protein 60 (HSP60) . Methods 40 four weeks male SD rats, weight 100 + 10 g, were randomly divided into 4 groups: the healthy control group (group C), atherosclerosis group (AS group), oral HSP60 group before building AS model (H1 group), oral HSP60 group after building AS model (H2 group), 10 rats in each group. AS model was made by high fat feed. At 180 days age, the rats were executed after obtaining the rats blood and myocardial. The gene and protein expression of rats myocardial VEGF and HIF ‐ 1 alpha were detected by reverse transcription ‐ polymerase chain reaction (RT‐PCR) and immunohistochemical. Results The VEGF gene expression in rats myocardial of group C, AS group, H1group and H2 group respectively are 0.374±0.039, 0.869±0.049, 0.443±0.031 and 0.583±0.041; the gene expression of HIF ‐ 1 alpha respectively are 0.455±0.056, 0.936±0.034, 0.553±0.048 and 0.625±0.063; the protein expression of VEGF respectively are 4.46±0.47, 9.17±0.58, 5.28±0.37 and 8.14±0.49; the protein expression of HIF ‐ 1 alpha respectively are 5.77±0.71,13.87±0.43,7.01±0.61and 0.45±0.80. Conclusion The gene and protein expression of VEGF and HIF ‐ 1 alpha in atherosclerotic rats myocardial enhanced compared with normal control group. Oral HSP60 can reduce the gene and protein expression of VEGF and HIF ‐ 1 alpha in myocardial, and reduce the degree of myocardial ischemia anoxic injury and vascular endothelial inflammatory response. Moreover, the earlier oral, the better results. Grant Funding Source : Supported by the Science and technology development projects in Shanxi Province (No. 2011004)