Wolbachia pipientis occurs in Aedes aegypti populations in New Mexico and Florida, USA

The mosquitoes Aedes aegypti (L.) and Ae. albopictus Skuse are the major vectors of dengue, Zika, yellow fever and chikungunya viruses worldwide. Wolbachia , an endosymbiotic bacterium present in many insects, is being utilized in novel vector control strategies to manipulate mosquito life history and vector competence to curb virus transmission. Earlier studies have found that Wolbachia is commonly detected in Ae. albopictus but sporadically detected in Ae. aegypti . In this study, we used a two-step PCR assay to detect Wolbachia in wild-collected samples of Ae. aegypti . The PCR products were sequenced to validate amplicons and identify Wolbachia strains. A Loop-mediated isothermal amplification (LAMP) assay was developed and used for detecting Wolbachia in selected mosquito specimens as well. We found Wolbachia in 85/148 (57.4%) wild specimens of Ae. aegypti from various cities in New Mexico and in 2/46 (4.3%) wild specimens of Ae. aegypti from St. Augustine, Florida. We did not detect Wolbachia in 94 samples of Ae. aegypti from Deer Park, Harris County, Texas. Wolbachia detected in Ae. aegypti from both New Mexico and Florida was the wAlbB strain of Wolbachia pipientis . A Wolbachia positive colony of Ae. aegypti was established from pupae collected in Las Cruces, New Mexico in 2018.