Efficient doubled haploid production in microspore culture of Zengcheng flowering Chinese cabbage (Brassica campestris L. ssp. chinensis [L.] Makino var. utilis Tsen et Lee)

Scientia Horticulturae(2019)

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摘要
•Microspore culture is used for rapid development of doubled haploids.•Microspore culture protocol was developed for Zengcheng flowering Chinese cabbage.•Adding right VcNa concentrations to a medium improved microspore embryogenesis.•High-yield and self-incompatible DH lines were identified for breeding.
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关键词
Zengcheng flowering Chinese cabbage,Microspore culture,L-ascorbic acid sodium salt,Embryogenesis,Doubled haploid lines
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