Efficient doubled haploid production in microspore culture of Zengcheng flowering Chinese cabbage (Brassica campestris L. ssp. chinensis [L.] Makino var. utilis Tsen et Lee)
Scientia Horticulturae(2019)
摘要
•Microspore culture is used for rapid development of doubled haploids.•Microspore culture protocol was developed for Zengcheng flowering Chinese cabbage.•Adding right VcNa concentrations to a medium improved microspore embryogenesis.•High-yield and self-incompatible DH lines were identified for breeding.
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关键词
Zengcheng flowering Chinese cabbage,Microspore culture,L-ascorbic acid sodium salt,Embryogenesis,Doubled haploid lines
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