Biotinylated fluorescent probe for the specific and quantitative determination of blood glucose

The quantitative detection of biomarkers in protein-rich samples using fluorescent probes is usually hampered by nonspecific fluorescence as a result of nonspecific probe-protein interactions. In this paper, we report a biotinylated fluorescent probe that is encapsulated in avidin protein, which can generate very specific fluorescence in blood serum by blocking out nonspecific dye-protein interactions. This approach was applied successfully to quantify glucose concentrations in blood serum.