3D Graphene/Copper Oxide Nano-flowers Based Acetylcholinesterase Biosensor for Sensitive Detection of Organophosphate Pesticides

•AChE-CS/3DG-CuO NFs could greatly amplify the electrochemical signal and provide favorable conditions for the detection of OPs.•This biosensor exhibited a wide linear relationship to malathion ranging from 1 ppt to 15.555 ppb and a low detection limit of 0.31 ppt.•The recovery rates of water samples were in the range from 94% to 106%, indicating an effective platform for pesticides detection.