Poly(butylene terephthalate) Fiber Assembly with Controllable Pore Size and Gradient Wettability: Potential in Simplifying Cell Culture Procedure

This study reports the scalable fabrication of a poly(butylene terephthalate) fiber assembly featured with controllable pore size and gradient wettability. Pore size is controlled via adjusting the throughput of melt blown process, while gradient wettability is achieved through single-sided plasma exposure and subsequent chitosan coating. When used in cell culture, the fiber assembly takes much less time in reaching a high cell collecting/releasing rate up to ≥99.5%, which is similar to that of the conventional centrifugal method. Other advantages of the fiber assembly, such as improved cell viability, reduced risk of contamination, and excellent reusability are also proved, leading us to believe its great potential in making the current cell culture procedure simpler and faster.