Abstract 251: Lna- Mediated Mir-92a Inhibition Induces Therapeutic Neovascularization in a Pig Model of Chronic Myocardial Ischemia

miRNA are post-transcriptional gene regulators which modulate RNA silencing and gene expression. In chronic myocardial ischemia, miRNA are altered and dysregulated. MiR92a inhibition in a mouse model of LAD occlusion led to improved neovascularisation and reduced infarct sizes. We aimed to investigate this therapeutic effect of miR92a- inhibition in a pig model of chronic myocardial ischemia. Methods: In pigs (n=5/group) reduction stent graft implantation in the circumflex artery led to a gradual occlusion of the vessel within 28 days (d28) after intervention. Using selective pressure-regulated retroinfusion the nucleic acid-modified antisense miR92a (LNA-92a; 5 mg/KG heart weight) was injected 28 days post implantation. The global myocardial function (Ejection Fraction (EF)) and the left end-diastolic pressure (LVEDP) were obtained at day 28 and 56. At day 56 after stent implantation regional myocardial function (subendocardial segmentshortning) as well as post mortem angiographies for collateral growth ...