Single layer graphene functionalized MEA for enhanced detection of neuronal network development

•Commercial multi-electrode-arrays (MEAs) well functionalized with single layer graphene (SLG) transferred via wet etching.•Spontaneous activity of neuronal networks on graphene was recorded from week 1 to week 4, up to complete network maturation.•Higher neuronal survival rate, higher adhered cell number and enhanced firing activity were detected on SLG versus control.•Neuronal network activity was detected earlier on graphene and a more synchronous behavior of the network was recorded.•Graphene functionalized MEAs showed an improved detection capability, possibly due to a better neuron/substrate coupling.