The advantages of next-generation sequencing technology in the detection of different sources of abscess

Abstract We reported the application of next-generation sequencing to identify a series of different sources of abscess cases indicating next-generation sequencing could offer unbiased sequencing and rapid diagnosis of pathogens. For the cases of having positive culture results, the metagenome analysis results were all consistent with those of conventional culture examination. Moreover, NGS could detect multiple pathogenic microorganisms and demonstrated dramatically decrease reads of identification pathogens in recovery phase. This technology provides a powerful ability for the rapid etiology diagnosis of patients with abscess and is expected to be advocated as one of the most valuable, supplemental tool in clinical diagnostic microbiology by offering unbiased sequencing and culture-free or culture-negative infections pathogen detection.