Record of alien Cinara (Cinara) cedri, (Hemiptera: Aphididae) Istria, Croatia

Aphid species Cinara cedri (Mimeur, 1936), the unusual species of the Croatian aphid fauna, was recorded in the area of Pula (Istria, Croatia) on October 23rd, 2012 (Figure 2,3). It was found in the southern part of the town on Cedrus libani tree. The species was identified by combined morphological and molecular approaches. Standardised fragment of the mitochondria! cytochrome c oxidase I gene (DNA barcode region) was used as a molecular marker. Molecular analysis supported morphological identification of specimens as Cinara cedri. Phylogenetic analysis placed Pula specimens within highly supported, monophyletic Cinara cedri Glade. In the overlaping region (629 bp) their sequences are identical to the sequence of Cinara cedri from China, while the pairwise genetic distance between them and 56 other Cinara species included in analysis were greater than 7.7 % (Figure 4 b). Identification through Barcode of Life Data Systems (BOLD) web platform (currently containing DNA barcode sequences of 99 species of genus Cinara), matched Pula specimens to Cinara cedri with 99.7 % probability of placement. Monitoring conducted during the time period of 2013-2015 in the wider area has not revealed either new populations of this species or the population increment at the original location.