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Influence of different immobilization techniques to improve the enantioselectivity of lipase from Geotrichum candidum applied on the resolution of mandelic acid

Wilson Galvão de Morais Júnior, Ariel Moura Maia,Pedro Alves Martins,Gloria Fernández-Lorente,Jose M. Guisan,Benevides C. Pessela

Molecular Catalysis（2018）

Cited 9|Views16
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Abstract
•We tested the immobilization of a pure Geotrichum candidum lipase on different supports.•The derivates were used as a biocatalyst in the hydrolysis of mandelic acid.•Enantiomeric excess was determined and evaluated.•The pH can totally switch the enantioselectivity of the enzyme.
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Key words
Geotrichum candidum lipase,Immobilization,Stabilization,Enantioselectivity,(R,S)-mandelic acid ethyl ester
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