Abstract 15513: miR-210 HypoxamiR Stimulates Neoangiogenesis in Response to Peripheral Ischemia

Background: MicroRNA-210 (miR-210) is induced by hypoxia in endothelial cells. The analysis of miR-210-loaded RISC complexes by transcriptomics identified many miR-210 targets involved in cell migration and angiogenesis. Accordingly, miR-210 expression increased endothelial tubulogenesis and VEGF-driven migration in vitro. Here, we investigated the regulation of angiogenesis by miR-210 in peripheral ischemia. Methods and Results: Transcriptomic analysis by RNA seq of hypoxic endothelial cells showed that MIR210 Host Gene was the most induced long noncoding RNAs (27±1 fold, adj pu003c1 E-76). Accordingly, its product miR-210 was also increased (35±5 fold; pu003c0.00001). In keeping with in vitro data, miR-210 was induced 2.9±0.6 fold (pu003c0.04) in ischemic muscles of a mouse model of hindlimb ischemia induced by femoral artery dissection. miR-210 role was investigated by the administration of miR-210 complementary LNA oligos (anti-210) and in doxycycline-inducible miR-210 transgenic mice (Tg-210). MiR-210 stimulated...