Nagarse treatment of cardiac subsarcolemmal and interfibrillar mitochondria accounts for inaccurate quantification of proteins
Journal of Molecular and Cellular Cardiology(2020)
摘要
Background: In the heart, subsarcolemmal (SSM), interfibrillar (IFM) and perinuclear mitochondria represent three subtypes of mitochondria. The most commonly used protease during IFM isolation is the nagarse, however, its effect on the detection of mitochondrial proteins is still unclear. Therefore, we investigated whether nagarse treatment influences the quantification of mitochondrial proteins.
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关键词
interfibrillar mitochondria accounts,cardiac subsarcolemmal,nagarse treatment,proteins
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