Investigation on Electrochemical Behavior and Catalytic Function of Glassy Carbon Electrode on the Basis of Magnetic Nano-particle with Simultaneous Incorporation of Myoglobin and Electron Mediator

As-prepared magnetic nano-composite with Fe 3 O 4 as core and carboxymethylated Chitosan as shell was proposed to act as matrix to co-immobilize electron relay and myoglobin by the means of chemical coupling. Subsequently Mb based bio-cathode was fabricated through the procedure of drop-casting in the presence of external magnet and was examined its performance in direct electron transferring and catalysis in electro-reduction of H 2 O 2 with spectrometric and electro-chemical methods. Analysis in results indicated adjacent complexations between hetero-atoms in polymer and cofactors in enzyme/redox site of electron mediator would pose great impact on spectroscopic and electrochemical features of immobilized protein. These interactions would alter the path of electron shuttle and mechanism of catalysis.