Detection of Trichomonas vaginalis using urethral , vaginal and cervical swabs with standard diagnostics from males and females in Canton Sarajevo

Objectives: Trichomoniasis is a non-viral sexually transmitted infection (STI) caused by the protozoan parasite Trichomonas vaginalis (TV). The aim of our study was to identify the most frequent TV isolates using urethral, vaginal and cervical swabs with standard diagnostics from males and females. Material and methods: The analysis of collected swabs (urethral, vaginal and cervical) was performed at the Department of Microbiology, Institute for Public Health of Canton Sarajevo, from June 2016 to March 2017. Microscopic evaluation of wet preparations and cultivation were demonstrated as the most common method for TV diagnostics. The urethral swabs were collected all together with vaginal and cervical in absolute value of 4784 from males and females. Results: T. vaginalis was identified in the frequency of 269 isolates. The highest frequency of positive isolates was in June (N=69) and July (N=45) in 2016 while the lowest rate was in January 2017 (N=8). There were 53 cases of total positive swabs among males and females from January to March 2017 in Canton Sarajevo and median age was 29 year interquartile range (25.0-37.5). Out of them 30 were males and 23 females with no statistical significance. Conclusions: T. vaginalis was identified from urethral, vaginal and cervical swabs with standard diagnostics in high frequency from males. Our data suggest that wet mount examination of fresh samples contribute to increase the sensitivity in the diagnosis of trichomoniasis.