Abstract 13.25 THE INFLUENCE OF ADIPOSE DERIVED STEM CELL ON NERVE REGENERATION

OBJECTIVE: The aim of the study was to assess the effect of autologous fat graft on nerve regeneration by creating a suitable experimental model. METHODS: The rat sciatic nerve was used, transected and primary neurorrhaphy was used on both hind legs, but on one side a processed fat graft was applied, surrounding the nerve. RESULTS: For the follow-up we used histological examination, at 4 and 10 weeks. The results showed increased and more organised neural regeneration on the side with the fat graft. CONCLUSIONS: The adipose-derived stem cell has clearly demonstrated her capacity to transdifferentiate, but the specific role played is not clear. We wanted to explore the direct effect of this cell on direct neurrorhaphy. We did not observe a direct differentiation on Schwann like cell, but mostly an antifibrotic and an antiinflammatory effect. References: 1. Raposio E, Caruana G, Bonomini S, Libondi G, A novel and effective strategy for the isolation of adipose-derived stem cells: minimally manipulated adipose-derived stem cells for more rapid and safe stem cell therapy. Plast Reconstr Surg. 2014 Jun;133(6):1406–9. 2. Shomari DL Zack-Williams, Peter E Butler, and Deepak M Kalaskar, Current progress in use of adipose derived stem cells in peripheral nerve regeneration. World J Stem Cells. 2015 Jan 26; 7(1): 51–64. 3. Patricia A. Zuk. The Adipose-derived Stem Cell: Looking Back and Looking Ahead. Mol Biol Cell. 2010 Jun 1; 21(11): 1783–1787. doi:10.1091/mbc.E09-07-0589