Water Permeability and Elastic Properties of an Archaea Inspired Lipid Synthesized by Click Chemistry

Author(s): Leriche, G; Manafirad, A; Nguyen, S; Bell, N; Patterson, JP; Thayumanavan, S; Yang, J; Dinsmore, AD; Gianneschi, NC | Abstract: Researchers report the synthesis of a new archaea-inspired tetraether lipid and study the mechanical elastic properties of membranes made from this bolalipid. To access structural diversity in the hydrophobic moiety of the lipids and to tune membrane properties, they have used used Huisgen 1,3-dipolar cycloaddition strategy to design and synthesize a tetraether lipid incorporating phytanyl side chains and polar 1,4 triazole rings known to change lipid packing. The researchers demonstrate the first example of the synthesis of phytanyl side chain incorporated tetraether lipids with 1,4-triazole rings. They also confirm the ability of lipids incorporating 1,4-triazole rings to form stable small and giant vesicles and examined their physical and mechanical properties using a micropipette aspiration technique.