Simultaneous Determination of Succinic Acid and Water-Soluble Vitamins by Ion-Pair High-Performance Liquid Chromatography
Journal of Analytical Chemistry(2018)
Abstract
A procedure is proposed for the determination of succinic acid, riboxin, nicotinamide, and riboflavin by ion-pair HPLC with UV detection. Owing to the addition of an ion-pair modifier to the mobile phase and the selection of gradient elution conditions, the optimal retention and resolution of peaks of the components to be determined are achieved. Specificity, linearity, accuracy, and precision of the developed procedure are proved on an example of the determination of active substances in the Cerebronorm® preparation.
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Key words
ion-pair HPLC,gradient elution,succinic acid,water-soluble vitamins,Cerebronorm®
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