Synthesis Of Manganese Oxide Embedded Carbon Nanofibers As Effective Biomimetic Enzymes For Sensitive Detection Of Superoxide Anions Released From Living Cells

Carbon nanofiber/manganese oxide (CNF-MnO) hybrid nanofibers are synthesized by calcination of potassium permanganate (KMnO4) loaded bacterial cellulose (BC) hydrogels. The chemical structure, morphology, performance, and application of CNF-MnO aerogels are characterized and studied. The results revealed that MnO nanoparticles are uniformly deposited on the surface of CNF which derived from BC hydrogels. An amperometric superoxide anions (O-2(center dot-)) sensor is fabricated by the immobilization of the CNF-MnO aerogels on a glassy carbon electrode, which displays a linear amperometric response with a high sensitivity of 76.2 mu A cm(-2) x 10(-3)m(-1) and a low detection limit of 1.2 x 10(-9)m in the concentration range of 5.0 x 10(-9)m - 2.5 x 10(-6)m. The successful detection of O-2(center dot-) released from cancer cells verifies the potential application in biomedical field.