Su1762 - Esophageal Submucosal Glands As a Source of Progenitor Cells Capable of Differentiating into Squamous or Barrett's-Like Columnar Cells

Background: Information for prediction factors of concurrent chemo-radiotherapy (CCRT) response has been limited.Primary 3-dimensional (3D) cultured cells have advantages in providing more physiologically relevant and predictive data for in-vivo response.Method: Primary 3D cell (spheroid) culture was performed using tumor tissues acquired from esophageal cancer before 1 st CCRT start.After 7days cultured, same sized spheroids were collected and were treated with 5-FU and 5Gy radiotherapy was provided.After 6days, primary cultured cells were stained and fluorescent images were captured.Clinical response was assessed after 4 th cycle CCRT.Clinical response was classified as complete remission (CR), partial remission (PR), and disease progression (PD).Results: A total of 26 esophageal cancer patients were enrolled.Final success rate of primary 3D cell culture was 77% (20/ 26).CCRT response in primary 3D cultured cell were evaluated in 20 cases.A total of 15 persons were followed up more than 4 cycles of CCRT and were analyzed.Clinical CR was observed in 9 persons and two persons showed clinical PR (n=4) or PD (n=2).Live activity was noted in less than 10% of primary 3D cultured cells in all patients with clinical CR and was observed in 30-40% of primary 3D cultured cells in all patients with clinical PD.Live activity was noted in less than 20-30% of primary 3D cultured cells in all patients with clinical PR Discussion: It takes 2weeks to evaluate the CCRT response in spheroid from tissue acquirement.High agreement between clinical response and response in spheroids was observed.The evaluation of CCRT response in spheroid will provide a good predictor of clinical CCRT response. Su1762