MULTIVARIATE CHEMOMETRIC MODELS APPLIED FOR SIMULTANEOUS DETERMINATION OF BROMHEXINE AND GUAIFENESIN IN PURE FORM AND IN PHARMACEUTICAL PREPARATION; A COMPARATIVE STUDY.

Experimental design of different synthetic mixtures of bromhexine and guaifenesin in different ratios were constructed .The zero-order absorption spectra of these prepared mixtures have been recorded and used for building four multivariate chemometric methods for simultaneous determination of bromhexine and guaiphenesin in their pure and pharmaceutical dosage forms. namely; partial least squares and artificial neural network have been applied for the quantitative analysis of the studied drugs. Alternatively, the application of genetic algorithm to partial least squares and artificial neural network has been done and greatly increased the precision and predictive ability of the methods. The four methods have been successfully applied for determination of both drugs in their pharmaceutical preparation without any preliminary separation steps.