DEVELOPMENT OF ATHEROSCLEROSIS-INDUCED CHRONIC PELVIC ISCHEMIA RAT MODEL FOR REPRODUCING THE DETRUSOR UNDERACTIVITY

You have accessJournal of UrologyUrodynamics/Lower Urinary Tract Dysfunction/Female Pelvic Medicine: Basic Research & Pathophysiology II1 Apr 2018MP38-01 DEVELOPMENT OF ATHEROSCLEROSIS-INDUCED CHRONIC PELVIC ISCHEMIA RAT MODEL FOR REPRODUCING THE DETRUSOR UNDERACTIVITY Myong Kim, Hwan Yeul Yu, Daehun Choi, Jyung Hyun Shin, Aram Kim, Chae-Min Ryu, Ju-Young Han, Dong-Myung Shin, and Myung-Soo Choo Myong KimMyong Kim More articles by this author , Hwan Yeul YuHwan Yeul Yu More articles by this author , Daehun ChoiDaehun Choi More articles by this author , Jyung Hyun ShinJyung Hyun Shin More articles by this author , Aram KimAram Kim More articles by this author , Chae-Min RyuChae-Min Ryu More articles by this author , Ju-Young HanJu-Young Han More articles by this author , Dong-Myung ShinDong-Myung Shin More articles by this author , and Myung-Soo ChooMyung-Soo Choo More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.1228AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES To our best knowledge, animal models reproducing detrusor underactivity (DUA) are scarce. Previous studies suggested that atherosclerosis, a common aging-associated disorder, has a role in the pathogenesis of lower urinary tract dysfunction, such as DUA. We tried to develop a rat model of atherosclerosis-induced chronic bladder ischemia and investigate the effect of chronic bladder ischemia on voiding behavior and bladder function. METHODS Adult male rats were divided into four groups. The arterial injury (AI) group underwent endothelial injury of the iliac arteries (AI-10, 10 times of injury at each iliac artery; AI-30, 30 times) and received a 2% cholesterol diet. The sham group underwent sham operation and received a 2% cholesterol diet. The control group received a regular diet. After 8 weeks, a metabolic cage study and cystometry were performed without anesthesia. Histological examination of the iliac arteries and the bladder was performed. The bladder was also processed for organ bath study. RESULTS The metabolic cage study showed that in the AI-30 group, micturition interval, voided volume, and residual volume were significantly increased. Cystometry showed that the frequency of reflex bladder contractions and micturition pressure were significantly lower in the AI-30 group. Histological study showed that in the AI group alone, atherosclerotic occlusion occurred in the iliac arteries as well as in the downstream bladder microvessels. Contractile responses of bladder strips to various stimuli in AI-30 group were significantly less than in sham group (Figure). CONCLUSIONS Pelvic arterial occlusive disease plus vascular endothelial dysfunction may cause progressive vascular damage resulting in bladder dysfunction that develops the detrusor underactivity © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e500 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Myong Kim More articles by this author Hwan Yeul Yu More articles by this author Daehun Choi More articles by this author Jyung Hyun Shin More articles by this author Aram Kim More articles by this author Chae-Min Ryu More articles by this author Ju-Young Han More articles by this author Dong-Myung Shin More articles by this author Myung-Soo Choo More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...