MP06-14 URINARY CELL-FREE MICRORNA RATIO WITH MIR-6124/MIR-4511 CAN DISCRIMINATE BLADDER CANCER FROM BENIGN HEMATURIA

You have accessJournal of UrologyBladder Cancer: Epidemiology & Evaluation I1 Apr 2018MP06-14 URINARY CELL-FREE MICRORNA RATIO WITH MIR-6124/MIR-4511 CAN DISCRIMINATE BLADDER CANCER FROM BENIGN HEMATURIA Won Tae Kim, Seok-Joong Yun, Xuan-Mei Piao, Ho Won Kang, Sung Pil Seo, Yong-June Kim, Sang Cheol Lee, and Wun-Jae Kim Won Tae KimWon Tae Kim More articles by this author , Seok-Joong YunSeok-Joong Yun More articles by this author , Xuan-Mei PiaoXuan-Mei Piao More articles by this author , Ho Won KangHo Won Kang More articles by this author , Sung Pil SeoSung Pil Seo More articles by this author , Yong-June KimYong-June Kim More articles by this author , Sang Cheol LeeSang Cheol Lee More articles by this author , and Wun-Jae KimWun-Jae Kim More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.206AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Although hematuria is the most common symptom in bladder tumor (BT), not all hematuria patients were diagnosed with BT. To identify a clinically applicable discriminating method of BT from non-cancer hematuric patients, the present study explored differences of urinary cell-free microRNAs (miRNAs) in urine samples of BT and non-cancer hematuric patients. METHODS Urine samples derived from 626 patients included 334 BT, 223 hematuria, and 69 pyuria in this study. A urine-based miRNA microarray analysis and real-time polymerase chain reaction (RT-PCR) suggested the presence of differentially expressed two urinary miRNAs in BT patients, and these were further validated in two cohorts using RT-PCR. RESULTS The expression level of miR-6124 was significantly higher in BT urine and miR-4511 was down-regulated in BT urine compared with non-cancer hematuria and pyuria. The ratio of miR-6124 to miR-4511 was considerably higher in BT urine than in non-cancer hematuria and enabled the discrimination of BT from non-cancer hematuric patients at a sensitivity of 79.1%, specificity of 70.3% with an area under the curve 0.761 (p<0.001). Furthermore, compared to voided urine cytology, urinary miR-6124 to miR-4511 showed better sensitivity for detecting BT in any grade (p<0.001). CONCLUSIONS Expression ratio of miR-6124/miR-4511 in urine could be a non-invasive promising tool for BT diagnosis among hematuric patients. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e57 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Won Tae Kim More articles by this author Seok-Joong Yun More articles by this author Xuan-Mei Piao More articles by this author Ho Won Kang More articles by this author Sung Pil Seo More articles by this author Yong-June Kim More articles by this author Sang Cheol Lee More articles by this author Wun-Jae Kim More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...