RT-MLPA as a New Tool for the Molecular Diagnosis of Allograft Rejection in Formalin Paraffin-embedded Endomyocardial Biopsies

Gene expression analysis of allograft tissue have been increasingly recognized as an interesting tool for the diagnosis of rejection as a companion to histopathology. This study evaluates in heart transplantation the diagnostic performance of Reverse Transcriptase-Multiplex Ligase-dependent Probe Amplification (RT-MLPA), a new technique of targeted gene expression analysis, suitable for formalin-fixed paraffin embedded (FFPE) tissue.