High-level production of trans -cinnamic acid by fed-batch cultivation of Escherichia coli
Process Biochemistry(2018)
摘要
•Trans-cinnamic acid has been widely used with various applications.•Trans-cinnamic acid pathway was reconstructed by optimization of PAL gene expression in E. coli.•With the engineered strain, 650 mg/L trans-cinnamic acid was produced in flask cultivation.•By the optimization of feeding solution, trans-cinnamic acid could be produced as high as 6.9 g/L in fed-batch cultivation.
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关键词
Trans-cinnamic acid,Escherichia coli,Fed-batch cultivation,Phenylalanine ammonia-lyase (PAL)
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