Nickel Metal Stress Induces Alterations In Expression Of Amylase And Acid-Phosphatase Isozymes In Cenchrus Ciliaris

When plants of a specific area grow in conditions that are far from optimum state, the first effect of such conditions is limitation in plant growth due to cellular damage. These sub optimal or imbalanced environmental conditions constitute stress. Conversely nature always tries to manage the unbalanced state because protective cellular, metabolic and molecular adaptations occur in plants to deal with these stresses that alter physiological reactions of the plants. Whole mechanistic details of these processes are still unknown. The present work focuses on the defensive role of two key enzymes amylase and acid phosphatase in plants under abiotic stress. Our study covers stress related alterations in expression pattern of isozymes of acid phosphatase (Apase) and amylase in Cenchrus ciliaris, a desert plant after treatment of Ni metal stress. The plants were grown hydroponically in the presence of different concentrations of Nickle (Ni) metal (0.3, 3, 10, 20 mg/ L). Results of Spectrophotometry and stain specific native PAGE showed that expression pattern of various isoforms of amylase and acid phosphatase varied with change in intensity of Ni metal stress in C. ciliaris. Thus Nickle (Ni) metal stress induces alterations in carbohydrate and phosphorus metabolism and C. ciliaris uses it as adaptive strategy to cope stress conditions. (C) 2017 Friends Science Publishers