Fast Decay Variants Of Red Fluorescent Genetically-Encoded Calcium Indicators
BIOPHYSICAL JOURNAL(2018)
Abstract
The time-course of intracellular Ca2+ transients is hard to assess due to buffering and signal integrating interactions. Genetically-encoded calcium indicators (GECI) have proven useful for monitoring Ca2+ transients in living cells and organisms. However, Ca2+ indicators with high Ca2+ affinity and slow decay kinetics themselves integrate Ca2+ signals, and furthermore may become saturated before peak [Ca2+] is reached. Thus, for more faithful tracking of rapid Ca2+ dynamics, probes with faster off-kinetics are required1,2. Red-fluorescent GECI have been developed with the view of multicolour imaging and optogenetic applications3. Here we report novel fast-decay variants of red-fluorescent genetically-encoded Ca2+ indicators jRGECO1a and jRCaMP1a3 with up to 8-fold (t1/2 of 6.4 ms) and 13-fold (t1/2 of 33 ms) faster in vitro decay kinetics (37 °C), respectively. Fast-decay jRGECO1a and jRCaMP1a variants retain comparable fluorescence brightness and dynamic range values to their parent proteins. The fluorescence dynamic range of the brighter mApple-based jRGECO1a variants is stable between pH 6.5 and 7.5, but declines above pH 7.5 to a Ca2+-independent fluorescent state. In contrast, the less bright jRCaMP1a variants, based on mRuby, are stable over the pH range of 6.5 to 10. Red-fluorescent GECI, like their green-fluorescent counterparts, are characterised by high cooperativity to Ca2+, and complex kinetic patterns of Ca2+-dependent fluorescence response with a limiting on-rate. However, the fast-decay variants of jRGECO1a and jRCaMP1a reveal 8-fold faster ATP-evoked Ca2+ transients compared to their parent proteins in HEK293T cells, showing the benefits of fast-decay red-fluorescent GECI indicators for monitoring Ca2+ dynamics in living cells. References: This work is funded by BBSRC grant BB/M02556X/1 to K.T. [1] Helassa et al., 2015 Scientific Reports, 5:15978. [2] Helassa et al., 2016 Scientific Reports, 6:38276. [3] Dana et al., 2016 eLife, 5:e12727.
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Key words
Fluorescent Chemosensors
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