The Effects of Diclofenac and Ibuprofen on HEK Cells in Cell Culture

Background: The studies show that the Diclofenac and Ibuprofen drugs influence cancer cells proliferation. The main aim of this study was to investigate the effects of Diclofenac and Ibuprofen on the viability of HEK cells in cell culture.Materials and Methods: In this laboratory experimental study, HEK cells were randomly divided into control group and groups exposed doses to 0.001,0/01,0/1,10,1 mg/ml of Ibuprofen and Diclofenac. The toxic effect of drugs was measured using the MTT assay method. The data were statistically analyzed between groups using ANOVA.Results: The viability of HEK cells exposed to 0.1, 0.01, 0.001 mg/ml of Ibuprofenwas not significant increase in compared with control group . And the viability of HEK cells exposed to 1, 10, 0.1, 0.01, 0.001 mg/ml of Diclofenac significantly decreased compared to control group. (Classification Pu003c0.01، Pu003c0.01، Pu003c0.01، Pu003c0.05، Pu003c0.01 (.Conclusion: Diclofenac and Ibuprofen in high doses destroy the HEK cells. Therefore, the use of these drugs in high doses is inappropriate.Keywords: Diclofenac, Ibuprofen, HEK, Viability