Label-free fluorescent detection of alkaline phosphatase with vegetable waste-derived green carbon probes
Sensors and Actuators B: Chemical(2018)
Abstract
•A fluorescent carbon probe to detect alkaline phosphatase was prepared by modified ultrasound irradiation from vegetable waste.•Unique interaction among Cu2+, carbon probe, and pyrophosphate was used for the label-free detection of alkaline phosphatase.•The carbon probe-based biosensor showed high selectivity and sensitivity.•The biosensor showed great performance to determine alkaline phosphate levels in human serum with excellent precision.
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Key words
Carbon materials,Recycling,Fluorescent probes,Alkaline phosphatase,Bioassay
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