Rapid, selective and stable HaloTag-LbADH immobilization directly from crude cell extract for the continuous biocatalytic production of chiral alcohols and epoxides
Reaction Chemistry and Engineering(2018)
Abstract
A strategy for biocatalyst immobilization in flow directly from the crude cell extract is described. The efficiency and the stability of the immobilized enzyme were demonstrated during the asymmetric reduction of a range of ketones. The cascade two-step chemo-enzymatic preparation of chiral epoxides was possible through the initial ketone bioreduction to an intermediate halohydrin followed by its intramolecular cyclization.
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Key words
continuous biocatalytic production,chiral alcohols,halotag-<i>lb</i>adh immobilization,crude cell extract
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