Integration of Glass Micropipettes with a 3D Printed Aligner for Microfluidic Flow Cytometer
Sensors and actuators A, Physical(2018)
Abstract
In this study, a facile strategy for fabricating a microfluidic flow cytometer using two glass micropipettes with different sizes and a 3D printed millifluidic aligner was presented. Particle confinement was achieved by hydrodynamic focusing using a single sample and sheath flow. Device performance was extracted using the forward and side-scattered optical signals obtained using fiber-coupled laser and photodetectors. The 3-D printing assisted glass capillary microfluidic device is ultra-low-cost, not labor-intensive and takes less than 10 min to fabricate. The present device offers a great alternative to conventional benchtop flow cytometers in terms of optofluidic configuration. (C) 2017 Elsevier B.V. All rights reserved.
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Key words
Flow cytometry,Hydrodynamic focusing,Micropipette,3D printing,Optofluidics
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