HPLC法测定冠心宁注射液中依地酸二钠的含量

Song Nana,Li Xiangming,Zhou Yongyan,Zhang Ruizhi,Ding Yanpu,Yang Qin,Sun Shengbin,Jiang Guozhi

Asia-Pacific Traditional Medicine（2022）

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Abstract

目的:建立冠心宁注射液中辅料依地酸二钠的含量测定方法.方法:采用XB-C18色谱柱;含3.0％甲醇和0.1％甲酸的水溶液(用40％四丁基氢氧化铵溶液调pH=3.5)为流动相;以258 nm为检测波长;流速1mL·min-1;柱温40℃.结果:依地酸二钠在20.00～100.00μg/mL(r=0.9999)质量浓度范围内呈良好的线性关系;平均回收率(n=6)为103.92％.结论:该方法简便,专属性强,重复性好,可作为冠心宁注射液中依地酸二钠的质量控制方法.

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