Bioelectronic Circuit On A 3d Electrode Architecture: Enzymatic Catalysis Interconnected With Photosystem I

Artificial light-driven signal chains are particularly important for the development of systems converting light into a current, into chemicals or for light induced sensing. Here, we report on the construction of an all-protein, light-triggered, catalytic circuit based on photosystem I, cytochrome c (cyt c) and human sulfite oxidase (hSOX). The defined assembly of all components using a modular design results in an artificial biohybrid electrode architecture, combining the photophysical features of PSI with the biocatalytic properties of hSOX for advanced light-controlled bioelectronics. The working principle is based on a competitive switch between electron supply from the electrode or by enzymatic substrate conversion.