Characterization of novel monoclonal antibodies against the MERS-coronavirus spike protein and their application in species-independent antibody detection by competitive ELISA

Shuetsu Fukushi,Aiko Fukuma,Takeshi Kurosu,Shumpei Watanabe,Masayuki Shimojima,Kazuya Shirato,Naoko Iwata-Yoshikawa,Noriyo Nagata,Kazuo Ohnishi,Manabu Ato,Simenew Keskes Melaku,Hiroshi Sentsui,Masayuki Saijo

Journal of Virological Methods（2018）

引用 35|浏览17

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摘要

•Novel monoclonal antibodies against MERS-CoV were produced and characterized.•Anti-MERS-CoV antibody detection system by competitive ELISA was developed.•The competitive ELISA was validated using sera taken from dromedary camels.

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关键词

MERS coronavirus,Epidemiology,Neutralizing antibody,Competitive ELISA

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