Recombination of 19 X-STR loci in Chinese Han three-generation pedigrees

The use of X chromosome STRs is very efficient for determining kinship in some complex paternity testing or forensic cases. Currently, a novel kit of 19 X-STR loci (DXS8378, DXS7423, DXS10148, DXS10159, DXS10134, DXS7424, DXS10164, DXS10162, DXS7132, DXS10079, DXS6789, DXS101, DXS10103, DXS10101, HPRTB, DXS6809, DXS10075, DXS10074 and DXS10135) was developed. The aim of this study was to investigate genetic linkage and recombination fractions of these 19 X-STR loci in 36 three-generation (grandpa-mother-son) families with 40 meiosis of the Chinese Han population. Linkage analysis and calculation of recombination between pairs of markers were carried out. Our data showed that the 19 X-STR loci were arranged in 6 linkage groups. In addition, 3 mutations, 3 intra-group and 52 inter-group recombination were obtained. In conclusion, this study indicated that the multiplex system of 19 X-STR loci can be used as a useful supplementary method in complex kinship analysis.