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623 The influence of photoirradiation on the circadian clock genes in human keratinocytes and melanocytes

JOURNAL OF INVESTIGATIVE DERMATOLOGY(2017)

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Abstract
Human circadian rhythm is organized in hierarchical structure which composed of central pacemaker in the suprachiasmatic nucleus (SCN) of the ventral hypothalamus and peripheral organs. Visible light enters the retina and stimulates SCN, and then the central master clock in the SCN communicates timing information to the skin via a combination sympathetic innervation and secreted hormones. On the other hands, ultraviolet (UV) B rays directly induces expression of the circadian clock genes, such as Per1, Clock and Bmal1 in cultured human keratinocytes. In this study, we investigated that the influence of visible light and UV on the circadian clock genes in human keratinocytes and melanocytes. Stratified keratinocytes and melanocytes were monochromatically irradiated at 280-310 nm using a multiwavelength irradiation spectral apparatus. In addition, melanocytes were irradiated at 365-940 nm using a light emitting diode (LED). 24 h after irradiation, total RNA was extracted and analyzed by Cap Analysis of Gene Expression (CAGE). In keratinocytes, Per1, Per2, Clock, Cry, Bmal1 and RORA were upregulated by 280-300 nm irradiation compared with sham irradiation. In melanocytes, Per2 was upregulated by 280-310 nm irradiation. There was no remarkable change in 365-940 nm irradiation. In conclusion, the circadian rhythm in the skin cells is regulated by UVB irradiation. Future outlook on research is to investigate that the effect of UVA and visible light exposure on clock gene expression in keratinocytes.
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Key words
circadian clock genes,human keratinocytes,photoirradiation
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