Green synthesis of silver nanoparticle and silver based chitosan bionanocomposite using stem extract of Saccharum officinarum and assessment of its antibacterial activity

Synthesis of nanoparticles and nanocomposites using green route is the limelight of modern nanotechnology. Herein, we demonstrate the synthesis of silver nanoparticle and silver based chitosan bionanocomposite using the stem extract of Saccharum officinarum . The absorbance peak at 460 nm in the UV-vis spectrum reveals the synthesis of silver nanoparticles using the stem extract of Saccharum officinarum . The size of the synthesized silver nanoparticle was in the range of 10-60 nm obtained from transmission electron microscope analysis (TEM). The presence of silver nanoparticles on the chitosan suspension was identified by scanning electron microscope (SEM), energy dispersive X-ray spectroscopic (EDS) and Fourier transform infrared spectroscopy (FTIR_ analysis. The antibacterial activity of the synthesized silver based chitosan bionanocomposite was evaluated against Bacillus subtilis (MTCC 3053), Klebsiella planticola (MTCC 2277), Streptococcus faecalis (ATCC 8043), Pseudomonas aeruginosa (ATCC 9027) and E. coli (ATCC 8739). The antibacterial activity of silver based chitosan bionanocomposite has remarkable scope in medicine, food packaging, textile and pharmaceuticals.